Part:BBa_K936020:Design

K206000+B0034+pelB+Cutinase+Polyhistidine Tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 210
  Illegal AgeI site found at 822
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There's no terminator so it needs to be used with a backbone that ends with a 3

Source

http://www.google.com/url?q=http%3A%2F%2Faem.asm.org%2Fcontent%2F78%2F5%2F1556.long&sa=D&sntz=1&usg=AFQjCNGjFC2TH89mPrYkWCAiYVccsHzdig